The picture above shows the comparison between VHH beads and conventional Antibody beads after immunoprecipitation experiments. VHH beads have obvious advantages and will not be affected by light chains or heavy chains.
Principle of Alpaca Anti-Tag Beads
Anti-Tag VHH beads are based on agarose beads or magnetic beads which are covalently coupled with alpaca VHHs with excellent performance and can pull down specific tag-fused protein and its interaction factors from tissues.
Principle of Alpaca Anti-Tag Beads
| Alpaca Anti-TAG Beads | Conventional Antibodies |
|---|---|
| Directly coupled to beads covalently | Need protein A/G coupled to beads first |
| No heavy and light chains in downstream interactions | Contamination of heavy and light chains in downstream applications |
| Acids and alkali resistance, tolerance to harsh conditions | IgG will limit the use of buffers, poor tolerance to some reagents |
| Short incubation time 5 – 30 minutes | Long incubation time |
| Low batch to batch variations | Not stable between batches |
| Typically, antibodies are not detectable in Western Blot | Antibody protein contamination in Western Blot |